Gene transcriptions/Elements/MYB recognitions
"Another class of cis-acting elements is represented by Boxes P and L from the parsley phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL) genes and the H-box from the bean chalcone synthase gene Chs15 [23,24]."[1]
"The combination of an [ACGT-containing element] ACE and a MRE confers light responsiveness to the CFI, F3H and FLS promoters."[2]
Consensus sequences
edit"These elements fit the type II MYB consensus sequence A(A/C)C(A/T)A(A/C)C, suggesting that they are MYB recognition elements (MREs)."[1]
The consensus sequence for the RRE is 5'-CATCTG-3'.[2]
The consensus sequence for the ACE is 5'-CACGT-3'.[2]
Flavonols
edit"Chalcone synthase (CHS), chalcone flavanone isomerase (CFI), flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS) catalyze successive steps in the biosynthetic pathway leading to the production of flavonols."[2]
In "Arabidopsis thaliana all four corresponding genes are coordinately expressed in response to light, and are spatially coexpressed in siliques, flowers and leaves."[2]
"Light regulatory units (LRUs) sufficient for light responsiveness were identified in all four promoters."[2]
A "R response element (RRE) was identified in the CHS promoter."[2]
"Only the ACE from the CFI promoter shows flanking sequences giving rise to a symmetrical G-box (Foster et al., 1994; Menkens et al., 1995) that also fits the consensus of the R motif (CANNTG; Blackwell and Weintraub, 1990; Bodeau and Walbot, 1996) which is recognized by [basic helix-loop-helix] BHLH factors."[2]
The gain-of-function for the FLS gene (MRE, ACE) lies between -97 and -42.[2]
For the F3H gene the ACE lies between -463 and -382 and the MRE lies between -99 and -63.[2]
For the CFI gene the ACE, MRE lie between -99 and -39.[2]
For the CHS gene the ACE, RRE, MRE lie between -106 and -69.[2]
See also
editReferences
edit- ↑ 1.0 1.1 Paul J Rushton and Imre E Somssich (August 1998). "Transcriptional control of plant genes responsive to pathogens". Current Opinion in Plant Biology 1 (4): 311-5. doi:10.1016/1369-5266(88)80052-9. http://arquivo.ufv.br/dbv/pgfvg/bve684/htms/pdfs_revisao/estresse/transcriptional.pdf. Retrieved 5 November 2018.
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 Ulrike Hartmann, Martin Sagasser, Frank Mehrtens, Ralf Stracke and Bernd Weisshaar (January 2005). "Differential combinatorial interactions of cis-acting elements recognized by R2R3-MYB, BZIP, and BHLH factors control light-responsive and tissue-specific activation of phenylpropanoid biosynthesis genes". Plant Molecular Biology 57 (2): 155–171. doi:10.1007/s11103-004-6910-0. http://pubman.mpdl.mpg.de/pubman/item/escidoc:1222162/component/escidoc:1222161/hartmann_weisshaar_pmb_2005.pdf. Retrieved 10 November 2018.