Gene transcriptions/Elements/MYB recognitions

"Another class of cis-acting elements is represented by Boxes P and L from the parsley phenylalanine ammonia-lyase (PAL) and 4-coumarate:CoA ligase (4CL) genes and the H-box from the bean chalcone synthase gene Chs15 [23,24]."[1]

A rosette of Arabidopsis thaliana with the inflourescence just emerging at the centre is imaged. Credit: Quentin Groom.{{free media}}

"The combination of an [ACGT-containing element] ACE and a MRE confers light responsiveness to the CFI, F3H and FLS promoters."[2]

Consensus sequences edit

"These elements fit the type II MYB consensus sequence A(A/C)C(A/T)A(A/C)C, suggesting that they are MYB recognition elements (MREs)."[1]

The consensus sequence for the RRE is 5'-CATCTG-3'.[2]

The consensus sequence for the ACE is 5'-CACGT-3'.[2]

Flavonols edit

"Chalcone synthase (CHS), chalcone flavanone isomerase (CFI), flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS) catalyze successive steps in the biosynthetic pathway leading to the production of flavonols."[2]

In "Arabidopsis thaliana all four corresponding genes are coordinately expressed in response to light, and are spatially coexpressed in siliques, flowers and leaves."[2]

"Light regulatory units (LRUs) sufficient for light responsiveness were identified in all four promoters."[2]

A "R response element (RRE) was identified in the CHS promoter."[2]

"Only the ACE from the CFI promoter shows flanking sequences giving rise to a symmetrical G-box (Foster et al., 1994; Menkens et al., 1995) that also fits the consensus of the R motif (CANNTG; Blackwell and Weintraub, 1990; Bodeau and Walbot, 1996) which is recognized by [basic helix-loop-helix] BHLH factors."[2]

The gain-of-function for the FLS gene (MRE, ACE) lies between -97 and -42.[2]

For the F3H gene the ACE lies between -463 and -382 and the MRE lies between -99 and -63.[2]

For the CFI gene the ACE, MRE lie between -99 and -39.[2]

For the CHS gene the ACE, RRE, MRE lie between -106 and -69.[2]

See also edit

References edit

External links edit