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UTPA STEM/CBI Courses/Microbiology/Genetic Engineering Using Bacteria

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Course Title: General Microbiology

Lecture Topic: Genetic Engineering in Bacteria

Instructor: Dr. Luis Materon

Institution: UTPA, Biology Department

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Challenge: There is currently a severe oil spill in the Gulf of Mexico that will affect wild life and business in the area. Task: Identify a gene that will degrade oil faster. The gene is to be inserted into a plasmid of an aquatic bacterial species such as Pelagibacter (The most abundant bacterial species in the ocean). The resulting recombinant bacteria having a new gene should accelerate the breakdown of oil on water and affected beaches. Adopt a protocol to isolate and insert a gene from Pseudomonas into Pelagibacter. Assess your contribution to reduce environmental damage from oil spill.


  • Backwards Design


Course Objectives

  • Primary Objectives- By the next class period students will be able to:
    • Understand
    • Discuss
    • Evaluate
    • Publish


  • Sub Objectives- The objectives will require that students be able to:
    • Consult protocols
    • Experiment with enzymes
    • Observe growth
    • Discuss results
    • Write report


  • Difficulties- Students may have difficulty:
    • Background knowledge in the molecular structure of DNA and other basic concepts
    • Getting access to lab after hours due to security regulations
    • Managing time
    • Reading results


  • Real-World Contexts- There are many ways that students can use this material in the real-world, such as:
    • Research-oriented job.
    • Teaching


Model of Knowledge

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  • Content Priorities
    • Enduring Understanding
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    • Important to Do and Know
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    • Worth Being Familiar with
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Assessment of Learning

  • Formative Assessment
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    • Homework (individual)
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  • Summative Assessment
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Legacy Cycle

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OBJECTIVE

By the next class period, students will be able to:

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The objectives will require that students be able to:

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THE CHALLENGE

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GENERATE IDEAS

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MULTIPLE PERSPECTIVES

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RESEARCH & REVISE

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TEST YOUR METTLE

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GO PUBLIC

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Pre-Lesson Quiz

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Test Your Mettle Quiz

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