Gene transcriptions/Boxes/HYs

A core responsive element is the hypertrophy region HY box between -89 and -60 nucleotides (nts) upstream from the transcription start site.[1]

Boxes Edit

A repeating sequence of nucleotides that forms a transcription or a regulatory signal is a box.

Consensus sequences Edit

"Deletion analysis by a series of 5′-deletion constructs identified the responsive region to RUNX-2 as being between −81 bp and −76 bp, containing a putative RUNX-2 binding sequence (TGAGGG), which is similar to that identified in the promoter region of human interleukin-3 (TGTGGG) (33)."[1] This suggests a consensus sequence of 3'-TG(A/T)GGG-5' on the template strand in the direction of transcription.

RUNX2 Edit

The gene RUNX2 "is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex."[2]

COL10A1 Edit

The gene COL10A1 "encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer."[3]

Human COL10A1, GeneID: 1300, has an HY box as the core responsive element.[1]

Hypotheses Edit

  1. A1BG is not transcribed by an HY box.

See also Edit

References Edit

  1. 1.0 1.1 1.2 Akiro Higashikawa, Taku Saito, Toshiyuki Ikeda, Satoru Kamekura, Naohiro Kawamura, Akinori Kan, Yasushi Oshima, Shinsuke Ohba, Naoshi Ogata, Katsushi Takeshita, Kozo Nakamura, Ung-Il Chung, Hiroshi Kawaguchi (January 2009). "Identification of the core element responsive to runt-related transcription factor 2 in the promoter of human type x collagen gene". Arthritis & Rheumatism 60 (1): 166-78. doi:10.1002/art.24243. PMID 19116917. Retrieved 2013-06-18. 
  2. "RUNX2 runt-related transcription factor 2 [ Homo sapiens (human) "]. Molecular & Cellular Proteomics. June 13, 2013. doi:10.1074/mcp.M113.035600. PMID 24309898. Retrieved 2020-10-09. 
  3. HGNC (June 9, 2013). "COL10A1 collagen, type X, alpha 1 [ Homo sapiens (human) ]". Bethesda, MD, USA: National Center for Biotechnology Information, U.S. National Library of Medicine. Retrieved 2013-06-18.

External links Edit