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Gene transcriptions/Boxes/MADS

Vineyard peaches shows leaves, branches and fruit. Credit: Tobias Maschler.{{free media}}

"The MADS-box encodes a novel type of DNA-binding domain found so far in a diverse group of transcription factors from yeast, animals, and seed plants."[1]

"The MADS-box comprises 180 nucleotides, encoding 60 amino acids [...] MADS is an acronym for the four DNA-binding proteins MCM1 [minichromosome maintenance gene 1], AGAMOUS [...], DEFICIENS [...], and SRF [serum response factor]."[1]

The "[Antirrhinum majus mutant squamosa (squa)] SQUA is a member of a family of transcription factors which contain the MADS-box, a conserved DNA binding domain."[2]

The "MADS-box is [...] AGAGGGAAAGTACAACTGAAGAGGATAGAGAACAAGATCAATAGACAGGTGACTTT CTCAAAGAGGAGAGGTGGATTGTTGAAAAAAGCTCATGAGCTCTCTGTGCTTTGTG ATGCTGAAGTGGCTCTTATTGTCTTCTCTAATAAGGGGAAGCTATTTGAGTATTCT ACTGAT",[2] which has 174 nucleotides (nts) and begins with the nucleotides for the amino acids RGK.[2] The six nucleotides following the MADS box are "TCTTGC"[2] which may be the additional six needed to get to 180 nts.

"RIN [Ripening Inhibitor] binds to DNA sequences known as the CA/T-rich-G (CArG) box, which is the general target of MADS box proteins (Ito et al., 2008)."[3]

PeachesEdit

"An AGC box (AGCCGCC) was found [from peach (Prunus persica L. Batsch cv. Loring)] between 886 and 892 bp upstream of the translation start site which has been shown in other ethylene-responsive PR genes to be a binding site for ethylene-responsive binding factor proteins (ERF proteins) (Ohme-Takagi and Shinshi, 1995; Sato et al., 1996; Jia and Martin, 1999; Fujimoto et al., 2000)."[4]

"The peach ACO1 does have an AGC box that has been found to bind ethylene responsive elements in response to pathogen infections (Ohme-Takagi et al., 2000; Rushton et al., 2002). Only the apple ACO1 also contains this sequence. In addition, both PpACO1 and the apple ACO1 have a MADS box transcription factor binding site (CarG) (Tilly et al., 1998), but none of the other ACO genes do. "[4]

See alsoEdit

ReferencesEdit

  1. 1.0 1.1 Günter Theißen, Jan T. Kim, Heinz Saedler (1 November 1996). "Classification and phylogeny of the MADS-box multigene family suggest defined roles of MADS-box gene subfamilies in the morphological evolution of eukaryotes". Journal of Molecular Evolution 43 (5): 484-516. doi:10.1007/BF02337521. http://link.springer.com/article/10.1007/BF02337521. Retrieved 2015-03-31. 
  2. 2.0 2.1 2.2 2.3 Peter Huijser, Joachim Klein, Wolf-Ekkehard Lonnig, Hans Meijer, Heinz Saedler and Hans Sommer (April 1992). "Bracteomania,an inflorescence anomaly, is caused by the loss of function of the MADS-box gene squamosa in Antirrhinum majus". The EMBO Journal 11 (4): 1239-49. PMID 556572. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC556572/pdf/emboj00089-0025.pdf. Retrieved 2015-04-01. 
  3. Masaki Fujisawa, Toshitsugu Nakano, Yoko Shima and Yasuhiro Ito (5 February 2013). "A large-scale identification of direct targets of the tomato MADS box transcription factor RIPENING INHIBITOR reveals the regulation of fruit ripening". The Plant Cell 25 (2): 371-86. doi:10.​1105/​tpc.​112.​108118. http://www.plantcell.org/content/25/2/371.short. Retrieved 2017-02-19. 
  4. 4.0 4.1 Hangsik Moon and Ann M. Callahan (2004). "Developmental regulation of peach ACC oxidase promoter–GUS fusions in transgenic tomato fruits". Journal of Experimental Botany 55 (402): 1519-28. doi:10.1093/jxb/erh162. http://jxb.oxfordjournals.org/content/55/402/1519.full. Retrieved 2014-05-07. 

External linksEdit