"Sequence comparison of the tomato RbcS-3A and RbcS-1 upstream regions shows three regions of strong homology, named the L [5'-AAATTAACCAAC-3'], I, and G boxes [...], contained within the fragment showing affinity for GBF. These homologies are highly significant, since these two genes diverged a long time ago (3) and do not share, in their upstream sequences, extensive regions of homology. All three boxes are found in tobacco (24) (Nicotiana tabacum) and Nicotiana plumbaginifolia (25) RBCS upstream sequences [...], while soybean upstream sequences lack a bona fide L box sequence (26)."[1]

An erect herb is photographed Nicotiana plumbaginifolia which will have white flowers. Credit: Yercaud-elango.{{free media}}

"In the tomato RbcS-3A promoter, a near-perfect box II homology (CATTTTCACT) is found just downstream of the L box [...]."[1]

Consensus sequences edit

"The sequence targeted by substitution P4 overlaps a sequence showing homology to the 'L' box (consensus 5'-AAATTAACCAA-3'), which is conserved in RBCS upstream sequences of both tomato and tobacco (Giuliano et al., 1988)."[2]

See also edit

References edit

  1. 1.0 1.1 G. Giuliano, E. Pichersky, V. S. Malik, M. P. Timko, P. A. Scolniks, and A. R. Cashmore (1 October 1988). "An evolutionarily conserved protein binding sequence upstream of a plant light-regulated gene". Proceedings of the National Academy of Sciences USA 85 (19): 7089-93. doi:10.1073/pnas.85.19.7089. http://www.pnas.org/content/pnas/85/19/7089.full.pdf. Retrieved 9 November 2018. 
  2. Robert G. K. Donald and Anthony R. Cashmore (1990). "Mutation of either G box or I box sequences profoundly affects expression from the Arabidopsis rbcS‐1A promoter". The EMBO Journal 9 (6): 1717-1726. doi:10.1002/j.1460-2075.1990.tb08295.x. https://onlinelibrary.wiley.com/doi/pdf/10.1002/j.1460-2075.1990.tb08295.x. Retrieved 8 November 2018. 

External links edit