Gene transcriptions/Boxes/HYs

A core responsive element is the hypertrophy region HY box between -89 and -60 nucleotides (nts) upstream from the transcription start site.^[1]

Boxes edit

A repeating sequence of nucleotides that forms a transcription or a regulatory signal is a box.

Consensus sequences edit

"Deletion analysis by a series of 5′-deletion constructs identified the responsive region to RUNX-2 as being between −81 bp and −76 bp, containing a putative RUNX-2 binding sequence (TGAGGG), which is similar to that identified in the promoter region of human interleukin-3 (TGTGGG) (33)."^[1] This suggests a consensus sequence of 3'-TG(A/T)GGG-5' on the template strand in the direction of transcription.

RUNX2 edit

The gene RUNX2 "is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex."^[2]

COL10A1 edit

The gene COL10A1 "encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer."^[3]

Human COL10A1, GeneID: 1300, has an HY box as the core responsive element.^[1]

Hypotheses edit

A1BG is not transcribed by an HY box.

References edit

↑ ^1.0 ^1.1 ^1.2 Akiro Higashikawa, Taku Saito, Toshiyuki Ikeda, Satoru Kamekura, Naohiro Kawamura, Akinori Kan, Yasushi Oshima, Shinsuke Ohba, Naoshi Ogata, Katsushi Takeshita, Kozo Nakamura, Ung-Il Chung, Hiroshi Kawaguchi (January 2009). "Identification of the core element responsive to runt-related transcription factor 2 in the promoter of human type x collagen gene". Arthritis & Rheumatism 60 (1): 166-78. doi:10.1002/art.24243. PMID 19116917. http://onlinelibrary.wiley.com/doi/10.1002/art.24243/full. Retrieved 2013-06-18.
↑ "RUNX2 runt-related transcription factor 2 [ Homo sapiens (human) "]. Molecular & Cellular Proteomics. June 13, 2013. doi:10.1074/mcp.M113.035600. PMID 24309898. https://www.mcponline.org/content/13/2/397.long. Retrieved 2020-10-09.
↑ HGNC (June 9, 2013). "COL10A1 collagen, type X, alpha 1 [ Homo sapiens (human) ]". Bethesda, MD, USA: National Center for Biotechnology Information, U.S. National Library of Medicine. Retrieved 2013-06-18.

External links edit

[Higashikawa-1] 1.0 ^1.1 ^1.2 Akiro Higashikawa, Taku Saito, Toshiyuki Ikeda, Satoru Kamekura, Naohiro Kawamura, Akinori Kan, Yasushi Oshima, Shinsuke Ohba, Naoshi Ogata, Katsushi Takeshita, Kozo Nakamura, Ung-Il Chung, Hiroshi Kawaguchi (January 2009). "Identification of the core element responsive to runt-related transcription factor 2 in the promoter of human type x collagen gene". Arthritis & Rheumatism 60 (1): 166-78. doi:10.1002/art.24243. PMID 19116917. http://onlinelibrary.wiley.com/doi/10.1002/art.24243/full. Retrieved 2013-06-18.

[RUNX2-2] "RUNX2 runt-related transcription factor 2 [ Homo sapiens (human) "]. Molecular & Cellular Proteomics. June 13, 2013. doi:10.1074/mcp.M113.035600. PMID 24309898. https://www.mcponline.org/content/13/2/397.long. Retrieved 2020-10-09.

[HGNC-3] HGNC (June 9, 2013). "COL10A1 collagen, type X, alpha 1 [ Homo sapiens (human) ]". Bethesda, MD, USA: National Center for Biotechnology Information, U.S. National Library of Medicine. Retrieved 2013-06-18.

[1]

[2]

[3]